From ben.crossett at sydney.edu.au Wed Sep 6 12:47:41 2017 From: ben.crossett at sydney.edu.au (Ben Crossett) Date: Wed, 6 Sep 2017 02:47:41 +0000 Subject: [Metabolomics] =?utf-8?q?FW=3A_Final_Reminder!_Webinar=3A_Let?= =?utf-8?q?=E2=80=99s_Chew_the_Fat_with_Orbitrap_MS_-_Enabling_Lipid_Metab?= =?utf-8?q?olic_Flux_Studies_and_Structural_Elucidation_of_Lipids_with_Ult?= =?utf-8?q?ra_High_Resolution_and_UVPD_Fragmentation?= In-Reply-To: <8bb055a69b9e4770ad291c6851bd28af@1913652004> References: <8bb055a69b9e4770ad291c6851bd28af@1913652004> Message-ID: <91043E40EC58C34BB59CEF09BC71C18701BDF6D906@ex-mbx-pro-04> Hi Guys, Gavin Reed is an excellent speaker and certainly knows his stuff so I would recommend registering for this webinar. The timing works well the delivery last week of a Q Exactive HFX which will be dedicated to lipidomics analysis. The second half of the talk is on ultraviolet photodissociation, a relatively new techniques but one which shows great promise and is certainly something on our shopping list of techniques we would like to see established here in Sydney. Kinds regards, Ben From: C&EN Webinars [mailto:cenwebinar at acs.org] Sent: Tuesday, 5 September 2017 11:13 PM To: Ben Crossett Subject: Final Reminder! Webinar: Let?s Chew the Fat with Orbitrap MS - Enabling Lipid Metabolic Flux Studies and Structural Elucidation of Lipids with Ultra High Resolution and UVPD Fragmentation Determining lipid turnover rates (lipid metabolic flux) based on stable isotope labeling and isotope dilution analysis remains a challenging task. Deuterated water is a commonly used reagent for these experiments as it is easily incorporated into metabolic pathways and simplifies the peak assignments compared to using heavier isotopes like 18O... If you have trouble viewing this email, read the online version. [C&EN] Final Reminder. Register Now. Let?s Chew the Fat with Orbitrap MS - Enabling Lipid Metabolic Flux Studies and Structural Elucidation of Lipids with Ultra High Resolution and UVPD Fragmentation [C&EN] September 07, 2017 [C&EN] USA 8:00am PDT, 11:00am EDT, 16:00 BST, 17:00 CEST [Rounded Rectangle: REGISTER NOW] Speakers [C&EN] Matt Mitsche, PhD., Postdoctoral Fellow, UT Southwestern Medical Center, TX [C&EN] Gavin E. Reid, PhD., Professor of Bioanalytical Chemistry, University of Melbourne [C&EN] Overview The first part of this webinar describes the use of ultra-high resolution MS to baseline-resolve these two peaks, greatly improving the selectivity and detection limit of this quantitative technique. The second part of the webinar discusses the introduction of a unique fragmentation mode, ultraviolet photodissociation (UVPD) for unambiguous characterization of various lipid classes thereby representing a major breakthrough for lipid research. Locating a double bond in the acyl chain of lipid molecules used to be impossible with soft fragmentation techniques such as CID or HCD... [Rounded Rectangle: Read More] Sponsors: [https://protect-au.mimecast.com/s/1RkoBZsX3YxJtZ?domain=images.acspubs.org] Key Learning Objectives [C&EN] Learn about ultra-high resolution MS that allow fluxomics analysis of lipids with sensitivity comparable to radioactivity tracing. [C&EN] Learn how applying UVPD fragmentation to lipids can aid in the localization of their site(s) of unsaturation, assignment of complex backbone and headgroup identities, and the differentiation of isomeric lipids within complex mixtures. Who Should Attend: Researchers focused on lipidomics and metabolomics analysis. Researchers using stable isotope-labeling studies for pathway analysis. Researchers performing structure elucidation and characterization of lipid species or other small molecule endogenous metabolites. [Rounded Rectangle: REGISTER NOW] [C&EN] [C&EN] [C&EN] [C&EN] Copyright ? 2017 C&EN Media Group. 1155 Sixteenth Street NW | Washington, DC 20036 You are receiving this message at ben.crossett at sydney.edu.au because you have shown interest in updates with the C&EN Media Group. Unsubscribe from similar announcements or unsubscribe from ALL ACS C&EN emails. [https://protect-au.mimecast.com/s/q0YwBQfp5m9Xsk?domain=app.acspubs.org] -------------- next part -------------- An HTML attachment was scrubbed... URL: -------------- next part -------------- A non-text attachment was scrubbed... Name: image003.png Type: image/png Size: 903 bytes Desc: image003.png URL: -------------- next part -------------- A non-text attachment was scrubbed... Name: image004.png Type: image/png Size: 1355 bytes Desc: image004.png URL: From david.sullivan at sydney.edu.au Wed Sep 6 16:11:58 2017 From: david.sullivan at sydney.edu.au (David Sullivan) Date: Wed, 6 Sep 2017 06:11:58 +0000 Subject: [Metabolomics] Metabolomics Digest, Vol 13, Issue 1 In-Reply-To: References: Message-ID: <64C3343478FACD48A11154AB172994E5010F6E311C@ex-mbx-pro-05> Thanks for the information. Two of the presentations relate to kinetic studies based on stable isotope administration to humans. In the past, we have been unable to satisfy local Ethics Committee requirements as to the certified sterile preparation of IV infusions for this purpose. If there is sufficient demand, it may be possible to encourage a local (Sydney) manufacturer to fill this niche. Regards David ________________________________________ From: Metabolomics [metabolomics-bounces at mailman.sydney.edu.au] on behalf of metabolomics-request at mailman.sydney.edu.au [metabolomics-request at mailman.sydney.edu.au] Sent: Wednesday, 6 September 2017 12:47 PM To: metabolomics at mailman.sydney.edu.au Subject: Metabolomics Digest, Vol 13, Issue 1 Send Metabolomics mailing list submissions to metabolomics at mailman.sydney.edu.au To subscribe or unsubscribe via the World Wide Web, visit https://mailman.sydney.edu.au/mailman/listinfo/metabolomics or, via email, send a message with subject or body 'help' to metabolomics-request at mailman.sydney.edu.au You can reach the person managing the list at metabolomics-owner at mailman.sydney.edu.au When replying, please edit your Subject line so it is more specific than "Re: Contents of Metabolomics digest..." Today's Topics: 1. Darren Creek - Centenary Institute Tuesday Seminar August 29th (Ben Crossett) 2. FW: Final Reminder! Webinar: Let?s Chew the Fat with Orbitrap MS - Enabling Lipid Metabolic Flux Studies and Structural Elucidation of Lipids with Ultra High Resolution and UVPD Fragmentation (Ben Crossett) ---------------------------------------------------------------------- Message: 1 Date: Tue, 22 Aug 2017 22:54:45 +0000 From: Ben Crossett To: "Metabolomics at mailman.sydney.edu.au" Subject: [Metabolomics] Darren Creek - Centenary Institute Tuesday Seminar August 29th Message-ID: <91043E40EC58C34BB59CEF09BC71C18701BDF41ACA at ex-mbx-pro-04> Content-Type: text/plain; charset="utf-8" Hi Folks, Like busses, you wait ages and then three turn up at once! Next Tuesday we have another metabolomics talk: 12.00pm to 1.00pm Tuesday 29th August, 2017 Dr Darren Creek Senior Lecturer, (Career Development Fellow) Monash Institute of Pharmaceutical Sciences "Discovering Mechanisms of Drug Action and Resistance with Metabolomics" The Centenary Institute Lecture Theatre, Level 6, Building 93, Royal Prince Alfred Hospital Grounds Lunch will be provided for attendees following the seminar. Darren will be around most of the day, if you would like to meet up with him contact: Anthony Don [mailto:a.don at centenary.org.au] About the speaker: Dr Darren Creek is a Senior Lecturer, NHMRC Career Development Fellow and head of the pharmaceutical metabolomics laboratory at the Monash Institute of Pharmaceutical Sciences. He completed his PhD at Monash University in 2007, and performed post-doctoral research in Uganda, Scotland and Australia working on antimalarial drug discovery and clinical trials, before focusing on metabolomics studies of tropical parasites.He developed several novel analytical methods and software tools for the metabolomics eld, and discovered novel pathways and drug mechanisms in protozoan parasites. Dr Creek?s laboratory currently uses metabolomics and proteomics to understand mechanisms of drug action and resistance for cancer and infectious diseases, with a major focus on African trypanosomiasis and malaria. Darren continues to develop novel metabolomics methods to advance the eld and is a Director of the international Metabolomics Society. About the seminar: Drug resistance poses a major threat to the management of infectious diseases, and new drugs are urgently required for many common infections.Unfortunately, the development of new drugs is often hampered by a lack of knowledge regarding the mechanisms of action and resistance of existing drugs,and of new lead compounds. Recent advances in post-genomic technologies, including metabolomics and proteomics, provide an opportunity for unbiased analysis of the impact of drug treatment on cellular biochemistry, allowing discovery of previously unanticipated mechanisms of drug action and resistance. We have used these technologies to discover the mode of action of over 100 compounds that target the causative agents of malaria and African trypanosomiasis. Furthermore, multi-omic analysis of malaria parasites resistant to the rst-, and last-line antimalarials, the artemisinins, revealed depleted levels of a key biomarker protein, and differential regulation of haemoglobin digestion and glutathione synthesis. These ndings provide opportunities for clinical translation of new biomarkers of drug resistance, and provide direct evidence of the resistance mechanisms which may be exploited to enhance the activity of these important medicines. -------------- next part -------------- An HTML attachment was scrubbed... URL: ------------------------------ Message: 2 Date: Wed, 6 Sep 2017 02:47:41 +0000 From: Ben Crossett To: "Metabolomics at mailman.sydney.edu.au" Cc: Stuart Cordwell Subject: [Metabolomics] FW: Final Reminder! Webinar: Let?s Chew the Fat with Orbitrap MS - Enabling Lipid Metabolic Flux Studies and Structural Elucidation of Lipids with Ultra High Resolution and UVPD Fragmentation Message-ID: <91043E40EC58C34BB59CEF09BC71C18701BDF6D906 at ex-mbx-pro-04> Content-Type: text/plain; charset="utf-8" Hi Guys, Gavin Reed is an excellent speaker and certainly knows his stuff so I would recommend registering for this webinar. The timing works well the delivery last week of a Q Exactive HFX which will be dedicated to lipidomics analysis. The second half of the talk is on ultraviolet photodissociation, a relatively new techniques but one which shows great promise and is certainly something on our shopping list of techniques we would like to see established here in Sydney. Kinds regards, Ben From: C&EN Webinars [mailto:cenwebinar at acs.org] Sent: Tuesday, 5 September 2017 11:13 PM To: Ben Crossett Subject: Final Reminder! Webinar: Let?s Chew the Fat with Orbitrap MS - Enabling Lipid Metabolic Flux Studies and Structural Elucidation of Lipids with Ultra High Resolution and UVPD Fragmentation Determining lipid turnover rates (lipid metabolic flux) based on stable isotope labeling and isotope dilution analysis remains a challenging task. Deuterated water is a commonly used reagent for these experiments as it is easily incorporated into metabolic pathways and simplifies the peak assignments compared to using heavier isotopes like 18O... If you have trouble viewing this email, read the online version. [C&EN] Final Reminder. Register Now. Let?s Chew the Fat with Orbitrap MS - Enabling Lipid Metabolic Flux Studies and Structural Elucidation of Lipids with Ultra High Resolution and UVPD Fragmentation [C&EN] September 07, 2017 [C&EN] USA 8:00am PDT, 11:00am EDT, 16:00 BST, 17:00 CEST [Rounded Rectangle: REGISTER NOW] Speakers [C&EN] Matt Mitsche, PhD., Postdoctoral Fellow, UT Southwestern Medical Center, TX [C&EN] Gavin E. Reid, PhD., Professor of Bioanalytical Chemistry, University of Melbourne [C&EN] Overview The first part of this webinar describes the use of ultra-high resolution MS to baseline-resolve these two peaks, greatly improving the selectivity and detection limit of this quantitative technique. The second part of the webinar discusses the introduction of a unique fragmentation mode, ultraviolet photodissociation (UVPD) for unambiguous characterization of various lipid classes thereby representing a major breakthrough for lipid research. Locating a double bond in the acyl chain of lipid molecules used to be impossible with soft fragmentation techniques such as CID or HCD... [Rounded Rectangle: Read More] Sponsors: [https://protect-au.mimecast.com/s/87W8BlULJbeOca?domain=images.acspubs.org] Key Learning Objectives [C&EN] Learn about ultra-high resolution MS that allow fluxomics analysis of lipids with sensitivity comparable to radioactivity tracing. [C&EN] Learn how applying UVPD fragmentation to lipids can aid in the localization of their site(s) of unsaturation, assignment of complex backbone and headgroup identities, and the differentiation of isomeric lipids within complex mixtures. Who Should Attend: Researchers focused on lipidomics and metabolomics analysis. Researchers using stable isotope-labeling studies for pathway analysis. Researchers performing structure elucidation and characterization of lipid species or other small molecule endogenous metabolites. [Rounded Rectangle: REGISTER NOW] [C&EN] [C&EN] [C&EN] [C&EN] Copyright ? 2017 C&EN Media Group. 1155 Sixteenth Street NW | Washington, DC 20036 You are receiving this message at ben.crossett at sydney.edu.au because you have shown interest in updates with the C&EN Media Group. Unsubscribe from similar announcements or unsubscribe from ALL ACS C&EN emails. [https://protect-au.mimecast.com/s/z4nRBmU5bvQJCV?domain=app.acspubs.org] -------------- next part -------------- An HTML attachment was scrubbed... URL: -------------- next part -------------- A non-text attachment was scrubbed... Name: image003.png Type: image/png Size: 903 bytes Desc: image003.png URL: -------------- next part -------------- A non-text attachment was scrubbed... Name: image004.png Type: image/png Size: 1355 bytes Desc: image004.png URL: ------------------------------ Subject: Digest Footer _______________________________________________ Metabolomics mailing list Metabolomics at mailman.sydney.edu.au https://mailman.sydney.edu.au/mailman/listinfo/metabolomics ------------------------------ End of Metabolomics Digest, Vol 13, Issue 1 ******************************************* From ben.crossett at sydney.edu.au Tue Sep 26 08:23:01 2017 From: ben.crossett at sydney.edu.au (Ben Crossett) Date: Mon, 25 Sep 2017 22:23:01 +0000 Subject: [Metabolomics] FW: Register Now! Webinar on Accurate and Confident Metabolic Profiling In-Reply-To: <9a2e801166d34bf0974506d3feeac594@1913652004> References: <9a2e801166d34bf0974506d3feeac594@1913652004> Message-ID: <91043E40EC58C34BB59CEF09BC71C18701BE570D13@ex-mbx-pro-04> Hi All, There is a seminar on the newly released Biocrates AbsoluteIDQ? p400 HR Kit coming up see below for details. The kit is sold via Millennium Scientific and Thermo (priced around US$5k, or US$60/sample...although it is proving harder than you think to get actual pricing). The MSCF will be evaluating one of these kits in the near future, so drop me a line if you want to give it a try. Cheers, Ben ________________________________ From: C&EN Webinars [cenwebinar at acs.org] Sent: 25 September 2017 23:13 To: Ben Crossett Subject: Register Now! Webinar on Accurate and Confident Metabolic Profiling The metabolic phenotype or profile of an individual provides a readout of the physiological status, disease risk, environmental exposure, drug and treatment response prediction. Reliable results are a must for targeted metabolite profiling when detecting and quantifying a predefined set of metabolites... If you have trouble viewing this email, read the online version. [C&EN] Final Reminder. Register Now. Accurate and Confident Metabolic Profiling - Combining a Standardized and Quantitative Targeted Assay with High Resolution Accurate Mass MS [C&EN] September 27, 2017 [C&EN] USA 8:00am PDT, 11:00am EDT, 16:00 BST, 17:00 CEST Register Now Speakers [C&EN] Barbara Wolf, PhD., Scientific Marketing & Biochemist, Biocrates Life Sciences AG [C&EN] Anastasia Kalli, PhD., Strategic Marketing Specialist, Thermo Fisher Scientific [C&EN] Overview The metabolic phenotype or profile of an individual provides a readout of the physiological status, disease risk, environmental exposure, drug and treatment response prediction. Reliable results are a must for targeted metabolite profiling when detecting and quantifying a predefined set of metabolites. [https://protect-au.mimecast.com/s/NX12BDU209Nqf4?domain=images.acspubs.org] Read More Sponsors: [https://protect-au.mimecast.com/s/Jb1WBnU24RJkfG?domain=images.acspubs.org] Key Learning Objectives [C&EN] Learn about targeted metabolomics, its associated challenges and how advanced workflows with kit development leads to more accurate and confident targeted analysis [C&EN] Meet the newly introduced Biocrates AbsoluteIDQ? p400 HR Kit, key benefits and analytical performance for targeted analysis of biologically relevant metabolites [C&EN] Discover how the AbsoluteIDQ? p400 HR Kit and Q Exactive Orbitrap MS family delivers a standardized, quality-controlled and reproducible quantitative assay in your lab Who Should Attend: Researchers interested in Metabolomics and Lipidomics Core Laboratory Scientists New users in mass spectrometry and metabolomics Researchers performing large-scale, epidemiological and disease-related metabolomics studies Register Now [C&EN] [C&EN] [C&EN] [C&EN] Copyright ? 2017 C&EN Media Group. 1155 Sixteenth Street NW | Washington, DC 20036 You are receiving this message at ben.crossett at sydney.edu.au because you have shown interest in updates with the C&EN Media Group. 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